Search results for "Primary cultures"
showing 3 items of 3 documents
Streptomyces coelicolor secretoma protects astrocytes from oxidative stress
2016
Streptomycetes are gram-positive bacteria that produce about 2/3 of pharmaceutically active secondary metabolites, such as antibiotics, and anti-tumor, immunosuppressive, antifungal and antiparasitic agents. In this study, we investigated the possible effects of Streptomyces coelicolor extracts and putative vesicular fraction on primary cultures of rat astrocytes, in both physiological and stressed conditions, induced by treatment with hydrogen peroxide. Briefly, crude extracts and putative vesicular fractions were prepared from two S.coelicolor strains (M145 wild-type strain and bold F166 strain), and used to treat primary astrocytes, which were then also treated with hydrogen peroxide. Da…
In vitro evidences of epithelial to mesenchymal transition in low cell-density cultured human fetal hepatocytes
2017
Abstract Culturing fetal hepatocytes in high cell-density allowed stabilization of the hepatocyte phenotype up to 8 weeks, including the maintenance of liver-specific functions. On the other hand, when cultured at low cell-density, fetal hepatocytes underwent morphological modifications and acquired fibroblastic morphology. Since a switch from E-cadherin to vimentin expression accompanied these changes, we hypothesized the occurrence of epithelial-to-mesenchymal transition when fetal hepatocytes were cultured at low cell-density. Changes in gene expressionsuch as up-regulation of fibrosis-related geneswere also observed, suggesting that the low cell-density culture system promoted the acqui…
Effect of extracellular vesicles from CSF of multiple sclerosis patients and healthy controls on astrocytes in culture
2017
INTRODUCTION Secretion of extracellular vesicles (EVs) and EV-mediated exchange of molecules among brain cells, under physiological conditions, has been described years ago. At the same time, ability of EVs to transfer proteins and nucleic acids to the surrounding cells, thus modifying their phenotypes, has been clearly demonstrated for tumor cells of different origins, including brain cancers. More recently, involvement of EVs in pathological processes affecting the Central Nervous System (CNS), and their capacity of transferring damaging cargoes have been also suggested. To explore these functions, we exposed primary cultures of astrocytes to EVs isolated from cerebral spinal fluid (CSF) …